2). Selleckchem Bioactive Compound Library Both surface expression measurement as well as real-time analysis of lung-derived CD11c+MHC class II+ DC confirmed the presence of various FcγR and revealed high RNA-levels of FcγRII and readily detectable mRNA of FcγRI and FcγRIII (Fig. 2). Similarly, all splenic DC subpopulations showed expression of the FcγR tested, with CD4−CD8− DC having slightly lower expression of FcγRI (Fig. 2A). Pulmonary macrophages expressed all tested FcγR on their surface (Fig. 2E). We hypothesized that increased antigen uptake by DC through FcγR could potentially lead to increased MHC class II-mediated T-cell proliferation, thereby facilitating allergic airway inflammation.
To compare whether OVA and anti-OVA IgG immune complexes (OVA-IC) influences antigen presentation by DC subsets in vitro, OVA and anti-OVA IgG were mixed at increasing ratios (from 4:1 to 1:4) and IC-formation confirmed using gel electrophoresis and mass spectrometry (data not shown). A ratio of 1:4 (OVA:anti-OVA IgG) led to readily detectable OVA-IC.
Hence, we used 25 μg/mL OVA or the same amount of OVA in immune-complexed form (OVA:anti-OVA IgG, 1:4) to pulse sorted spleen-derived DC subsets. The cells Ponatinib were then co-cultured with CFSE-labeled OT-II cells and antigen presentation was assessed by measuring T-cell proliferation, visualized as a progressive dilution of the CFSE fluorescent marker. CD4+CD8− DC and CD8−CD4− DC, but not CD8+CD4− DC, led to significantly increased T-cell proliferation
when pulsed with OVA-IC, as compared to OVA alone. This effect was completely abrogated when DC deficient for FcR γ-chain where used indicating the specificity of this effect (Fig. 3A and B). Similarly, experiments using low-endotoxin OVA (EndoGrade™ OVA) in combination with anti-OVA IgG revealed significantly augmented T-cell stimulation by splenic DC. Alternatively, splenic DC from TLR4-deficient Morin Hydrate mice likewise led to a highly significant increase in T-cell proliferation when pulsed with OVA-IC as compared to OVA alone, suggesting no considerable contribution of LPS-contamination of OVA (data not shown). In order to better define the relevance of our observation for allergic airway hyperresponsiveness, we then purified CD11c+MHCII+ DC from the lungs of the respective mice, pulsed the cells with OVA or IC and then used them to stimulate CFSE-labeled OT-II cells. Again, T-cell proliferation doubled when using lung DC from B6 mice, in a manner similar to splenic DC, but no such effect was observed when FcγR-deficient lung DC were used (Fig. 3A). Exposure of sorted CD11c+MHCII+ lung DC to OVA-IC led to IL-6 and TNF-α secretion (Fig. 3C) and up-regulation of the co-stimulatory molecule CD86 on BM-derived DC (BMDC) (Fig. 3D).