cannonballus mycelium from culture. Cultivars Nabijani, Sfidak khatdar, Sfidak bekhat, Ghandak, Mollamosai, Chappat, Hajmashallahi and Shadgan were moderately resistant to M. cannonballus but all other melon cultivars were moderately to highly susceptible (HS) to this pathogen. A second screening was performed for resistance to M. cannonballus under greenhouse conditions. In the second screening, cultivars Nabijani, Sfidak khatdar, Sfidak bekhat, Ghandak, Mollamosai, Chappat, Hajmashallahi and Shadgan were moderately resistant to M. cannonballus. To examine the melon resistance
mechanism against M. cannonballus, the activities of total phenol, total protein and peroxidase in HIF inhibitor two melon cultivars Nabijani (as resistant) and Khaghani (as susceptible)
were determined at 0, 24, 48 and 72 h after inoculation. Inoculated resistant cultivar roots had always higher content of total phenol, Barasertib total protein and peroxidase than the corresponding inoculated susceptible cultivar roots. The results indicated that there was a relationship between resistance in Nabijani and accumulation of total phenol, total protein and peroxidase. “
“Curtoviruses cause severe damage to tomatoes and peppers. Functional field resistance to curtoviruses in these plants is desirable but difficult to produce and difficult to screen for because it is time-consuming and resistance could be achieved by developing resistance either to the virus or to insect feeding. To improve and speed curtovirus resistance testing in tomato (Solanum lycopersicum) and pepper (Capsicum annuum) plants, two puncture
methods were developed and compared to leafhopper inoculation and feeding preference assays. The two puncture methods were adapted to introduce a modified Agrobacterium tumefaciens plasmid carrying a recombinant curtovirus into the meristem tissue of tomato plants and into newly germinated chile pepper seedlings. The puncture techniques were used to screen for resistance to curtoviruses in chile pepper and tomato breeding lines and varieties. Similarly, the peppers and tomatoes were assayed for curtovirus resistance using leafhopper inoculation and feeding preference, which Protein kinase N1 was assessed by stylet sheath staining. Virus infection by puncture and leafhopper feeding was monitored using PCR and ELISA. ELISA was performed using an antibody to bacterially expressed coat protein. While pepper cvs Tabasco, NuMex Las Cruces cayenne and New Mexico 6-4 were infected using both puncture and leafhopper inoculation methods, New Mexico 6-4 had higher infection rates than the other two cultivars. Stylet sheath staining results suggest that leafhoppers prefer to feed on New Mexico 6-4 rather than Tabasco and NuMex Las Cruces cayenne. Eight tomato cultivars were infected using meristem removal injection inoculation.