A detailed description of the experimental and control group procedures can be found in Appendix 1 (see the eAddenda for Appendix 1). Treatment was planned to result in 60 hours of positioning and 51 hours of NMES/TENS. All procedures

were performed by the local trial coordinator or instructed nursing staff. Nursing staff monitored compliance to the intervention by logging each session on a record sheet, which was always kept in the vicinity of the participant’s bed. During the first 8 weeks of the trial, prescription of pain and spasticity medication as well INCB024360 ic50 as content of physical and occupational therapy sessions for the arm were also monitored. The primary outcome measures were passive range of arm motion and pain in the hemiplegic shoulder. All goniometric assessments were performed by two observers using a fluid-filled goniometera.

Inter-observer reliability of this technique was high (de Jong et al 2012). The presence of shoulder pain was checked using the first (yes/no) question of the ShoulderQ (Turner-Stokes and Jackson 2006). The secondary outcome measures were timing and severity of poststroke shoulder pain, performance of real-life passive and basic daily active arm activities, hypertonia and spasticity, arm motor control and shoulder subluxation. All measurements were carried out in the same fixed order by the same two trained Panobinostat assessors. Every effort was made to motivate participants to undergo all planned measurements even after withdrawal from the study. Passive range of shoulder external rotation, flexion and abduction, elbow extension, forearm supination, wrist extension with extended and flexed fingers were assessed because these movements often develop restrictions in range as a result else of imposed immobility, with muscle contractures causing a typical flexion posture of the hemiplegic arm. The (entire) ShoulderQ was administered in participants who indicated that

they had shoulder pain. This questionnaire assesses timing and severity of pain by means of eight verbal questions and three vertical visual graphic rating scales. We were primarily interested in the answer to the (verbal) question How severe is your shoulder pain overall? (1= mild, 2 = moderate, 3 = severe, 4 = extremely severe) and pain severity measured at rest, on movement, and at night using the 10-cm vertical visual graphic rating scales. The ShoulderQ is sensitive ( Turner-Stokes and Jackson 2006) and responsive to change in pain experience ( Turner-Stokes and Rusconi 2003). Performance of basic functional activities of daily life involving the passive arm was assessed using the Leeds Adult/Arm Spasticity Impact Scale ( Ashford et al 2008).