No significant differences were found for total energy (366 ± 226 kcals) and % kcals CHO (57.9 ± 21.2%), % kcals fat (27.1 ± 16.0%), and % kcals PRO (15.0 ± 8.5%) in the meal consumed in the 24 hours prior to each supplement session. A significant main effect Selleck mTOR inhibitor of supplement was found for % kcals PRO (F(3,24) = 4.08, p < 0.05), such that % kcals PRO consumed before the CHO-CHO supplement was significantly greater than % kcals PRO consumed before the CHO-P supplement (19.2 ± 9.3% vs. 16.1 ± 12.5%, p = 0.042). No significant difference
was found for minutes since last meal consumed prior to each supplemental session (133.8 ± 133.4 minutes). No significant differences
were found for amount of aerobic exercise occurring in the 24 hours prior to each supplemental AZD5153 cost session (53.3 ± 67.9 min). Environmental factors (temperature [11.9 ± 7.2 °C]; percent relative humidity [57.8 ± 12.5%]; and wind speed [0.6 ± 0.5 mph]) were not significantly different between supplemental sessions. A main effect of time was found for RPE and HR (p < 0.001). RPE and HR increased at all points measured (4.7 ± 0.7; 9.7 ± 0.9, F(1,24) = 395.49; 84.4 ± 14.5 bpm, 166.0 ± 8.3 bpm, 178.8 ± 7.4 bpm, F(2,48) = 581.08). No significant differences in time to complete the run (PLA = 88.6 ± 11.6 min; CHO = 89.1 ± 11.3 min; CHO-P = 89.1 ±11.8 min; CHO-CHO = 89.6 ± 11.9 min) (Figure 1), or sprint to finish (PLA = 8.3 ± 1.2 min; CHO = 8.2 ±1.2 min; CHO-P = 8.2 ± 1.0 min; CHO-CHO = 8.4 ± 1.5 min) (Figure 2) was found among supplemental session. The effect size between any of the supplements and PLA on endurance performance was very small (d = 0.1).
Effect size between PLA and CHO, CHO-P, and CHO-CHO supplements learn more during the 19.2 km run, favoring PLA, was 0.06, 0.059, 0.1, respectively. In addition, for the last 1.92 km of the Orotidine 5′-phosphate decarboxylase course, the effect size between PLA and CHO and PLA and CHO-P, favoring the caloric supplements, was 0.08; effect size between PLA and CHO-CHO, favoring the PLA, was 0.07. Figure 1 Time to complete 19.2 km time trials for each supplement (M ± SD). N = 12; CHO = Carbohydrate; CHO-P = Carbohydrate-Protein; CHO-CHO = Double Carbohydrate; PLA = Placebo. Figure 2 Time to complete final 1.92 km sprint to the finish (M ± SD). N = 12; CHO = Carbohydrate; CHO-P = Carbohydrate-Protein; CHO-CHO = Double Carbohydrate; PLA = Placebo. Discussion The use of CHO-P supplementation during exercise, as opposed to CHO supplementation, is a rising trend among endurance athletes. Previous research has found mixed outcomes regarding CHO-P supplementation and endurance performance enhancement, with all investigations conducted within controlled laboratory settings [5–14].