J Invertebr Pathol 2003,84(2):96–103.PubMedCrossRef 20. Koch H, Schmid-Hempel P: Socially transmitted gut microbiota protect bumble bees against an intestinal parasite. P Natl Acad Sci USA 2011,108(48):19288–19292.CrossRef 21. Olofsson TC, Vasquez A: Detection and identification of a novel lactic acid bacterial flora within the honey stomach of the honeybee Apis mellifera. Curr Microbiol 2008,57(4):356–363.PubMedCrossRef 22. Vasquez A, Forsgren E, Fries I, Paxton RJ, Flaberg E, Szekely L, Olofsson TC: Symbionts as Major Modulators of Insect Health: Lactic Acid Bacteria LY3039478 price and Honeybees. PLoS One 2012,7(3):e33188.PubMedCrossRef 23. Pruesse E, Quast C, Knittel K,

Fuchs BM, Ludwig WG, Peplies J, Glockner FO: SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res 2007,35(21):7188–7196.PubMedCrossRef 24. Ludwig W, Strunk O, Westram R, Richter L, Meier H, Yadhukumar ,

Buchner A, Lai T, Steppi S, Jobb G, et al.: ARB: a software environment for sequence data. Nucleic Acids Res 2004,32(4):1363–1371.PubMedCrossRef 25. Mattila HR, Rios D, W-S VE, Roeselers G, Newton ILG: Characterization of the active microbiotas associated with honey bees reveals healthier and broader communities when colonies are genetically diverse. PLoS ONE 2012, 7:e32962.PubMedCrossRef 26. Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann DNA/RNA Synthesis inhibitor M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, et al.: Introducing mothur: open-source,

platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 2009,75(23):7537–7541.PubMedCrossRef 27. McDonald D, Price MN, Goodrich J, Nawrocki EP, DeSantis TZ, Probst A, Andersen GL, Knight R, Hugenholtz P: An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria Glutamate dehydrogenase and archaea. ISME J 2012,6(3):610–618.PubMedCrossRef 28. Euzeby JP: List of bacterial names with standing in nomenclature: A folder available on the Internet. Int J Syst Bacteriol 1997,47(2):590–592.PubMedCrossRef 29. Lan Y, Wang Q, Cole JR, Rosen GL: Using the RDP classifier to predict taxonomic novelty and reduce the search space for finding novel organisms. PLoS One 2012,7(3):e32491.PubMedCrossRef 30. Moran NA, Hansen AK, Powell E, Sabree ZL: Distinctive gut microbiota of honey bees assessed using deep sampling from individual worker bees. PLoS One 2012,7(4):e36393.PubMedCrossRef Competing interests The authors declare that they have no competing interest. Authors’ contributions ILGN conceived of the study, implemented the bioinformatics, analyzed resultant data, and drafted the manuscript. GR provided bioinformatics tools, participated in the analysis of the data, and helped to draft the manuscript. All authors read and approved the final manuscript.