CpG-ODN can suppress apoptosis of macrophages via TLR9 through PKB/Akt/FOXO pathway [22], since macrophages and T cells play an important role in anti-tumor immune, our study showed CpG-ODN suppresses apoptosis through FasL/Fas pathway, maybe PKB/Akt/FOXO is another way in anti-apoptosis anti-cancer therapeutic strategies of CpG-ODN. Currently, treatment of HCC relies on surgery, conventional chemotherapy, and radiation

therapy at clinic. Other therapeutic strategies, such as an antibody targeting the specific molecules, are currently in trials. DNA-based drugs, such as CpG-ODN and antisense ODN, are regarded as a new alternative therapy for the brain tumors [23]. The www.selleckchem.com/products/ABT-888.html regulation of the complex signaling pathways in tumors has been a new strategy for the rational FRAX597 research buy design of anticancer strategies. Escaping from immune surveillance and being resistant to apoptosis triggers play an important role in the progression and metastasis of tumors. Our results indicated that CpG-ODN down-regulated the FasL expression in HepG2 cells and Fas in Jurkat cells,

and suppressed the HepG2 cells-mediated caspase-dependent apoptosis of Jurkat cells. Conceivably, CpG-ODN treatment may be a promising strategy for the intervention of HCC. Acknowledgements We thank Dr. Lihua Hu, Department of Laboratory & Institute of Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, for her helpful comments on this manuscript. References 1. Vicari AP, Caux C, Trinchieri selleck inhibitor G: Tumour escape from immune surveillance through dendritic cell inactivation. Semin Cancer Biol 2002, 12:33–42.PubMedCrossRef 2. Gratas C, Tohma Y, Barnas C, Taniere P, Hainaut P, Ohgaki H: Up-regulation of Fas (APO-1/CD95) ligand and down-regulation of Fas expression in human esophageal cancer. Cancer Res 1998, 58:2057–62.PubMed 3. Wu JD, Higgins LM, Steinle A, Cosman D, Haugk K, Plymate SR: Prevalent Ureohydrolase expression of the immunostimulatory MHC class I chain-related molecule is counteracted by shedding in prostate cancer. J Clin Invest 2004, 114:560–8.PubMed 4. Roman

M, Martin-Orozco E, Goodman JS, et al.: Immunostimulatory DNA sequences function as T helper-1-promoting adjuvants. Nat Med 1997, 3:849–54.PubMedCrossRef 5. Sparwasser T, Vabulas RM, Villmow B, Lipford GB, Wagner H: Bacterial CpG-DNA activates dendritic cells in vivo: T helper cell-independent cytotoxic T cell responses to soluble proteins. Eur J Immunol 2000, 30:3591–7.PubMedCrossRef 6. Heckelsmiller K, Beck S, Rall K, et al.: Combined dendritic cell- and CpG oligonucleotide-based immune therapy cures large murine tumors that resist chemotherapy. Eur J Immunol 2002, 32:3235–45.PubMedCrossRef 7. Okamoto M, Sato M: Toll-like receptor signaling in anti-cancer immunity. J Med Invest 2003, 50:9–24.PubMed 8. Wooldridge JE, Weiner GJ: CpG DNA and cancer immunotherapy: orchestrating the antitumor immune response. Curr Opin Oncol 2003, 15:440–5.PubMedCrossRef 9.