Conversely, these authors found higher liver glycogen values in animals fed ad libitum, suggesting that the influence of dietary restriction on the content of this substrate is dependent on the tissue analysed. ACP-196 in vivo In this regard, further studies are needed to determine the changes caused by dietary restriction on the mechanisms of glycogen synthesis and utilisation in different tissues. The differences in

the levels of muscular glycogen could influence aerobic and Dabrafenib anaerobic capacity in animals, as determined using the lactate minimum test. However, there were no significant differences in the anaerobic threshold values between the groups, demonstrating that the diets and their form of administration did not influence the aerobic capacity of the animals. In addition, the loads corresponding to the anaerobic threshold in relation to body weight (4.5%) are similar to those reported by previous studies that used eutrophic rats [18, 32, 33] ARAÚJO et al., 2007). However, the animals in the ALD group showed lower lactate concentrations values. This

finding, together with the lower quantities of glycogen in the ad libitum groups, is consistent with those reported by Voltarelli, Gobatto and Mello [33], who observed lower lactate concentrations values in glycogen-depleted animals when comparing anaerobic BMS345541 threshold determined using lactate minimum test in a group of fed animals and a group of animals subjected to glycogen depletion. The animals in the ALD group showed the same characteristics observed in humans subjects during a lactate minimum test after glycogen depletion, i.e., the intensity corresponding to the minimum lactate concentration was not influenced by a reduction

in glycogen stores; however, the lactate concentrations were significantly lower upon depletion [20]. Further, the lactate concentrations and time to exhaustion values may have been influenced by the density of the animals ADAMTS5 in the ALD group, since these animals had an increase in body weight and body fat. Araújo, Araújo, Dangelo, et al. [34] demonstrated that the anaerobic threshold in obese animals, as determined using maximal steady state lactate levels, may be higher than that in well-nourished animals, attributing these findings to the lower density of these animals in an aquatic environment. Thus, in our study, the intensity at the same workload may have been underestimated for animals that have higher levels of fat [35], resulting in the lower lactate concentrations values and higher time to exhaustion values seen in the ALD group. Therefore, more studies are needed to normalise the variables related to the increased loads used in lactateminimum test as a function of the body density of the animals.