Nevertheless, three genera, Fusarium/Gibberella, Myrothecium, Pestalotiopsis/Pestalosphaeria and Microsphaeropsis/Paraphaeosphaeria, identified by Rocha et al. (2011) were not represented among our isolates even though the samples had the same origin of a rubber plantation in Bahia. The physiological state of the leaves

from which the endophytes were isolated, i.e. dry versus fresh leaves, could certainly have influenced the diversity of the recovered endophytic population. Among the specific genera that we found compared to Rocha et al. 2011, several species are known BMS-907351 solubility dmso to degrade wood, such as Xylaria sp. or Hypoxylon sp. (Chaparro et al. 2009). This suggested that our study was selective for species associated with senescent plant material. Supporting this hypothesis, Promputtha et al. (2002) showed that the stage of leaf decomposition in Magnolia liliifera had an important impact on the diversity of endophyte populations. An important result of our study is the identification of four C. cassiicola isolates. This is the first report of endophytic C. cassiicola in Hevea brasiliensis. C. cassiicola is primarily known as a pathogen affecting more than 300 plant species (http://​nt.​ars-grin.​gov/​fungaldatabases/​ (Farr and Rossman 2011)). However, C. cassiicola was also reported as an endophyte of Quercus ilex

(Collado et al. 1999), Aegle marmelos (Gond et al. 2007), Magnolia liliifera (Promputtha et al. 2007) and several other trees from GF120918 datasheet tropical forests (Suryanarayanan

et al. 2011). The fungus has also been observed as a saprotroph on cucumbers, tomatoes, papaya (Kingsland 1985), Bambusa spp. and Dendrocalamus spp. (Hyde et al. 2001), Ischyrolepis subverticella (Lee et al. 2004) and Magnolia liliifera (Promputtha et al. 2007, 2010; Kodsueb et al. 2008). However, many other plants can support C. cassiicola growth as a pathogen, endophyte or saprotroph (Dixon et al. 2009). Our results demonstrate that, even though outbreaks Fenbendazole of CLF disease have not yet occurred in South America, C. cassiicola is present in rubber trees on the American continent. Are endophytic C. cassiicola isolates latent pathogens or latent saprotrophs? Many species known to cause disease in plants are regularly isolated from asymptomatic tissues and are therefore also classified as endophytes (Kumar and Hyde 2004; Photita et al. 2004, 2005). Whether these are different subspecies or the same strain able to switch from one lifestyle to another is usually unknown. In the case of cacao (Rojas et al. 2010), haplotype subgroups were distinguished among check details Colletotrichum gloeosporioides isolates that were preferentially associated with either symptomatic or asymptomatic interactions. However, the isolates collected from asymptomatic tissues were not tested for pathogenicity.