High FDG uptake of extraabdominal L/N was found on 2 patients (inguinal and supraclavicular L/N). They were diagnosed as benign L/N enlargement. High FDG uptake on bone was found at 4 patients. 3 patients of them showed bone metastasis in liver CT and 1 patient who had metastasis to mandible diagnosed as both adrenal metastasis without
staging change. 1 patient showed high uptake on prostate and confirmed http://www.selleckchem.com/products/Roscovitine.html as benign nodule on biopsy. 1 patient with abdominal muscle metastasis was detected on both liver CT and 18F-FDG PET-CT. Skin metastasis was suspected on 1 patient and was confirmed as false positive high uptake of FDG. Conclusion: 27 patients of 160 patients were suspected as extrahepatic metastasis on 18F-FDG PET-CT. However there was no change on staging and treatment after 18F-FDG PET-CT because most of them were already suspected on liver CT or confirmed as false positive on biopsy and on other confirmative examinations. Key Word(s): 1. 18F-FDG PET-CT; 2. HCC; 3. metastasis; 4. stage; Presenting Author: CHEN WU Additional Authors: YUXIU YANG Corresponding Author: CHEN WU, YUXIU YANG Affiliations: Henan Provincial Hospital Objective: It has been found that cyclindepenr Kinase 5 (CDK5) has high correlation with kinds of nerve system diseases, lung cancer, prostatic carcinoma, and hepatic tumor. By analyzing the abnormal
expression of CDK5 in blood plasma of patients with hepatocellular carcinoma (HCC), we can make a study of the relativity between CDK5 and HCC, and then explore the clinical significance of this relativity in diagnosis of HCC. Methods: The selleck sample collected included the plasma of 60 patients with hepatocellular carcinoma, 40 patients with cirrhosis and 60 healthy controls. In the experiment, the enzyme linked immunosorbent assay (ELISA) and MCE PCR techniques with SYBR Green I fluorescent quantization can be used respectively
to measure the expression levels of CDK5 protein and mRNA of the sample. Then the sensitivity and specificity could be calculated, and the relationship between the expression of CDK5 and clinical pathological parameters was analyzed. In addition, data were analyzed by SPSS 17.0 software. Results: The expression level of CDK5 protein in plasma of patients with HCC was higher than those in patients with cirrhosis and healthy controls (p < 0.05), and it had no-relationship with sex, age, size, the value of AFP, the size of the tumor and the TMN stages (p > 0.05). At the same time, compared with hepatocirrhosis patients and healthy controls, the expression level of mRNA of CDK5 in plasma of HCC patients was found higher (p < 0.05), and it had no-relationship with sex, age, the value of AFP, the size of the tumor and the TNM stage (p > 0.05). Conclusion: CDK5 showed high relative with hepatocellular carcinoma, and CDK5 in plasma can be considered as an assistant sign of tumor to diagnose hepacellular carcinoma. Key Word(s): 1. HCC; 2. CDK5; 3. ELISA; 4.