Triterpenic glycoside content of some clones was evaluated confirming that
active compound content is highly variable. Implications of our results for further genetic studies and set-up breeding program are discussed. (C) 2013 Elsevier B.V. All rights reserved.”
“Sevoflurane is used widely during lung cancer surgery. However, the effect of sevoflurane on the invasion and migration of lung carcinoma cells remains unclear. The aims of this study were to explore the role of matrix metalloproteinase (MMP)-2 and MMP-9 in the effect of sevofluane on the invasion and the role of fascin and ezrin on the effect of sevofluane on the migration of human lung Mizoribine adenocarcinoma A549 cells. We also investigated whether sevoflurane regulates the expression of these molecules through the p38 mitogen-activated protein kinase (MAPK) signaling pathway.
The invasion of cells was
evaluated using the Transwell invasion assay, and the migration of cells was determined using the buy CX-6258 wound healing assay. The expression of MMP-2, MMP-9, ezrin, fascin, and phospho-p38 MAPK in cells was determined by western blotting.
A significant inhibition of cell invasion and migration was found in A549 cells which had been treated with sevoflurane. The data also revealed that sevoflurane could decrease the phosphorylation level of p38 MAPK, which is involved in the downregulation of MMP-2, MMP-9, fascin, and ezrin expression, accompanied by JQ-EZ-05 cell line a concomitant inhibition of the invasion and migration of A549 cells. SB203580, a p38 MAPK inhibitor, augmented the downregulation of the expression of these proteins.
The
anti-invasion effect of sevoflurane on A549 cells was associated with a downregulation of both MMP-2 and MMP-9 expression, while the anti-migration effect was associated with a downregulation of both fascin and ezrin expression. These effects could occur partly as a result of inactivation of the p38 MAPK signaling pathway.”
“Although the significance of osteoporosis is well known worldwide, the number of patients undergoing therapy for this disease corresponds to a fraction only of the optimal proportion. In many countries, patients at a high risk of fracture – including those who have in fact sustained a typical fracture – do not receive therapy at all. Unawareness of the population, difficulties in accessing specialist care, as well as insufficient communications all contribute to the fact that a substantial percentage of patients discontinue drug therapy prematurely. Using information from a survey conducted in a club of osteoporotic patients, this paper discusses the achievements and substantiates the existence of patient associations, as well as emphasizes the need for establishing new patient clubs in Hungary.”
“Porcelain gallbladder is regarded as a risk factor of gallbladder cancer.