Nucl Acids Symp Ser 1999, 41:95–98. 77. Feil EJ, Li BC, Aanensen DM, Hanage WP, Spratt BG: eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes
from multilocus sequence typing data. J Bacteriol 2004, 186:1518–1530.CrossRefPubMed 78. CDC: Standardized molecular subtyping of foodborne bacterial pathogens by pulsed-field gel electrophoresis: a manual Atlanta, GA: National Center for Infectious Diseases 1996. (updated 2000). 79. Sambrook J, Russell DW: Molecular cloning. A laboratory manual Third Edition New York: Cold Spring Harbor Laboratory Press 2001. 80. National Center for Biotechnology Information[http://www.ncbi.nlm.nih.gov] Authors’ contributions MW performed most of the MLST and part of the PFGE data, helped in the generation MG-132 in vitro and analysis of the data from the accessory genes, and helped to draft the manuscript. MBZ provided the isolates, performed the antimicrobial susceptibility MAPK inhibitor tests and most of the PFGE data, participated in the study design, performed the statistical analysis and helped to draft the manuscript. EC started the conception of the study, participated in its design and coordination, and helped to draft the manuscript. MFM participated in the performance of the laboratory work, such as the PCR assays, plasmid extraction procedures and southern hybridizations. JJC participated in the initial design of the epidemiological
study and in the conception
of this study. CS conceived and performed most of the work on the analysis of the accessory genome, helped in the generation of the MLST data, and drafted the manuscript. All authors read and approved the final manuscript.”
“Background Chlamydiosis and Q fever, two zoonosis, are widely distributed around the world. Their importance is related not only to the economic losses in animal production, but also to risks posed to humans [1, 2]. They are caused respectively by strictly intracellular and Gram negative bacterium Chlamydophila and Coxiella burnetii. Although C. burnetii and Chlamydophila belong to phylogenetically unrelated species [3], they show some similarities in their interaction with the host and pathogenesis of the infection [4]. Chlamydiaceae family is composed of nine species recognized within the two genera of Chlamydia and Chlamydophila [5] which are associated Dichloromethane dehalogenase with a large variety of diseases in animals and humans including abortion, pneumonia, gastroenteritis, encephalomyelitis, conjunctivitis, arthritis and sexually transmitted diseases [6]. The reservoir is large and includes many wild and domestic mammals but domestic ruminants such as sheep, cattle and goat represent the most frequent source of human infection. Two species of the genus Chlamydophila cause diseases in ruminants, Chlamydophila abortus (formerly Chlamydia psittaci serotype 1) and Chlamydophila pecorum (formerly Chlamydia pecorum). Cp.