A number of recent review articles have addressed the importance of sandfly-borne phleboviruses in Western Europe (Charrel et al., 2005, Cusi et al., 2010, Depaquit et al., 2010, Nicoletti et al., 1996 and Maroli et al., 2013). In the present paper, special attention has been given to data from Eastern Europe and from Middle-Eastern and North African (MENA) countries. The genus Phlebovirus, (family Bunyaviridae), contains nine viral species (Sandfly fever Naples, Salehabad, Rift valley fever, Uukuniemi, Bujaru, Candiru,

Chilibre, Frijoles, Punta Toro), and several tentative species, as defined in the 9th Report of the International Committee for Taxonomy of Viruses (ICTV) ( Plyusnin et al., 2011). In the Old World, Uukuniemi virus is transmitted by ticks, Rift valley fever virus is transmitted by mosquitoes, Sandfly fever Naples and Salehabad viruses are transmitted by sandflies. Sandfly-borne phleboviruses buy Etoposide are transmitted by Lutzomyia flies in the New MI-773 mw World and by Phlebotomus flies in the Old World. The dichotomy is absolute. Considering sandfly-borne phleboviruses of the Old World, the ICTV recognizes at present two viral species (Sandfly fever Naples, Salehabad) and two tentative

species (Sicilian, Corfu) ( Fig. 1). All members of the genus Phlebovirus have a trisegmented, negative-sense, single-stranded RNA genome. The L, M and S segments encode the RNA-dependent RNA polymerase, the viral envelope glycoproteins and in the case of the S segment, both the viral nucleocapsid protein (N) and a nonstructural protein (Ns) ( Liu et al., 2003, Suzich et al., 1990 and Xu et al., 2007). The single stranded RNA segments are known to have high Montelukast Sodium mutation rates due to the lack of proofreading activity of the viral polymerase which may result in genetic drift due to individual accumulated point mutations. RNA viruses are known to replicate as quasispecies populations, a situation favoring development of mutants with modified phenotypic characteristics, and possibly higher virulence and modified properties.

Single stranded RNA viruses are known to undergo major evolutionary events due to recombination; this has been demonstrated for many viruses in the Bunyaviridae family. The organization of the genome in the form of three segments renders possible genome reassortment (genetic shift), an important evolutionary event characterized by the exchange of genetic material between two distinct virus strains during co-infection of a single eukaryotic cell, resulting in the creation of a chimeric virus potentially exhibiting unique characteristics including virulence potentiation. Sandflies in the genera Phlebotomus, (Rondani and Berté, 1840); Sergentomyia, (França and Parrot, 1920); and Lutzomyia, (França, 1924) belong to the order Diptera, family Psychodidae, and subfamily Phlebotominae.